Download e-book for iPad: Animal Cell Culture: A Practical Approach by John R. W. Masters

By John R. W. Masters

ISBN-10: 0199637962

ISBN-13: 9780199637966

This re-creation of Animal phone tradition covers new or up-to-date chapters on phone authentication, serum-free tradition, apoptosis assays, FISH, genetic amendment, scale-up, stem phone assays, three-d tradition, tissue engineering and cytotoxicity assays. precise protocols for a large choice of tools give you the middle of every bankruptcy, making new method simply available. all people operating in organic and clinical learn, even if in academia or a advertisement association, practicing telephone tradition will gain enormously from this booklet.

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Gaush, C. , Hard, W. L, and Smith, T. F. (1966). Proc. Soc. Exp. Biol. , 122, 931. 35. Graham, F. , Russell, W. , and Nairn, R, (1977). J. Gen. Virol, 36, 59. 36. Knowles, B. , Howe, C. , and Aden, D. P. (1980). Science, 209, 497. 37. Gey, G. , Coffman, W. , and Kubicek, M. T. (1952). , 12, 364. 38. Puck, T. T. and Marcus, P. I. (1955). Proc. Natl. Acad. Sri. USA, 41, 432. 39. Gluzman, Y. (1981). Cell, 23, 175. 40. , Wright, W. , and Loveless, J. D. (1977). J. Natl. Cancer Inst, 58, 209. 41. Soule, H.

Mycoplasma are not visible to the naked eye, and, while they can affect cell growth, their presence is often not obvious. It is important to test for mycoplasma at regular intervals (every one to three months) as they can seriously affect almost every aspect of cell metabolism, antigenicity, and growth characteristics. Several tests have been proposed, but the fluorescent DNA stain technique of Chen is the most widely used (see Chapter 3), although it is less sensitive than the PCR-based and culture methods.

Pyrex) is preferred because soda-lime glass releases alkali into the medium and needs to be detoxified (by boiling in weak acid) 34 SCALING-UP OF ANIMAL CELL CULTURES before use. After repeated use glassware can become less efficient for cell attachment, but efficiency can be regained by treatment with 1 mM magnesium acetate. After several hours soaking at room temperature the acetate is poured away and the glassware is rinsed with distilled water and autoclaved. ii. Plastics Polystyrene is the most used plastic for cell culture, but polyethylene, polycarbonate, Perspex, PVC, Teflon, cellophane, and cellulose acetate are all suitable when pre-treated correctly.

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Animal Cell Culture: A Practical Approach by John R. W. Masters

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